Fig. 8: agenT-797 acts via soluble factors to rescue partially exhausted T cells. | Nature Communications

Fig. 8: agenT-797 acts via soluble factors to rescue partially exhausted T cells.

From: A phase 1/2 clinical trial of invariant natural killer T cell therapy in moderate-severe acute respiratory distress syndrome

Fig. 8

Supernatant from agenT-797 co-culture with A375-CD1d-GFP cells was collected after 24 h and added to coculture assays for antigen-exposed NY-ESO TCR + T cells as shown in the schematic in (A). B Impact of agenT-797 supernatant (sup) on cytotoxicity of Round 2 antigen exposed T cells against target cells. Left panel: killing curves over 5 days. Datapoints represent mean with SD (n = 3). Right panel: Cytotoxicity as measured by area under the curve (AUC). Bars represent mean with SEM (n = 3). C Impact of agenT-797 supernatant an activation of Round 2 antigen-exposed T cells measured by CD25 + CD69+ expression after 24 h of co-culture. Bars in bar graph represent mean with SD (n = 3). Colors represent conditions as in (B). B, C Supernatant from activated agenT-797 significantly improved the cytotoxicity and activation of partially exhausted T cells. Column means compared using Šídák’s multiple comparisons test. P values adjusted for multiple comparisons.

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