Fig. 1: LIF overexpression induces cachexia in the TgLC mice.
From: Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia in mice
A The strategy to generate LIF-tgflox/+ (TgL) mice. The mouse LIF gene, preceded by the CAG promoter and a transcriptional STOP cassette, was knocked into the Rosa26 locus (CAG-STOP-LIF-eGFP-Rosa26TV) using the CRISPR/Cas9 system. B The generation of TgL/R26-CreERT2 (TgLC) mice. Right panel: the genotyping analysis of TgL and TgLC mice by PCR. All mice with the same genotype have similar results. C Serum LIF levels in TgL (n = 6) and TgLC mice (n = 10) with TAM injection measured by the ELISA assay. D Mouse body weight post TAM injection in TgL mice (n = 6) and TgLC mice (n = 14). E Fat and lean mass loss post TAM injection in TgL (n = 10) and TgLC (n = 8) mice. Body composition was measured by EchoMRI. F Representative H&E images of muscle and WAT tissues from TgL and TgLC mice with TAM injection. At least three independent biological replicates were performed. G–M Mice were housed in Promethion metabolic cages. Mice were injected with TAM at the first light cycle (n = 4–10/group). Shaded regions represent the dark cycle from 19:00 pm to 7:00 am. Values are hourly means. Energy balance (G), food intake (H), total energy expenditure (TEE) (I), oxygen consumption (VO2) (J), carbon dioxide production (VCO2) (K), Respiratory exchange ratio (RER) (L) and locomotor activity (M) of TgL and TgLC mice post TAM injection were measured. N The serum levels of albumin, blood urea nitrogen (BUN), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT) reflecting kidney and liver functions in TgL and TgLC mice at 3 days after TAM injection for albumin and 9 days after TAM injection for other parameters (n = 3/group). O Kaplan-Meier survival curves of mice. The day of TAM injection was denoted as D0. Data are presented as mean ± SEM for (D, E), and as mean ± SD for (C, G–N). N.D. non-detectable. Each dot represents an individual mouse. Both female and male mice were used. For G–N: Two-tailed Student’s t-test; for D, E two-way ANOVA followed by Sidak’s multiple comparison test; and for O two-tailed Kaplan-Meier survival analysis. Source data are provided as Source Data file.
