Fig. 4: LIF overexpression downregulates the PPARα signaling pathway.
From: Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia in mice
A RNA-seq results showing gene expression levels in the liver from TAM-injected TgLC (n = 4) and TAM-injected TgL mice (n = 3). The number of identified genes and DEGs were shown (left). The DEGs were shown in the Heatmap (right). B KEGG analysis of DEGs by the DAVID database. C GSEA enrichment plots for lipid metabolism (left) and PPAR signaling pathway (right). D RNA-seq results showing the expression levels of genes (PPARa, PPARd, PPARg, PPARGC1a and PPARGC1b) encoding for five PPAR family members (n = 3 for TgL group, n = 4 for TgLC group). E Heatmap of PPARα target genes among the DEGs in the liver from TgLC and TgL mice with TAM injection. F KEGG map of PPARα signaling pathway. DEGs were mapped to the “PPARα signaling pathway”, according to the “PPARs signaling pathway” map in KEGG with some modifications. DEGs are colored in blue. G, H Validation of expression levels of PPARa and some of its target genes after LIF overexpression by qPCR assays (G; n = 4/group) and Western-blot assays (H). At least three independent biological replicates were performed. All data are presented as mean ± SD. Each dot represents an individual mouse. Both female and male mice were used. For D, G: two-tailed Student’s t-test. Source data are provided as Source Data file.
