Fig. 3: The components analysis of sgPik3cg-DHP/DGA-NVs. | Nature Communications

Fig. 3: The components analysis of sgPik3cg-DHP/DGA-NVs.

From: Bacterial protoplast-derived nanovesicles carrying CRISPR-Cas9 tools re-educate tumor-associated macrophages for enhanced cancer immunotherapy

Fig. 3

a, b Size distribution and abundance of DNA and RNA from E. coli, protoplast, OMVs and sgPik3cg-DHP/DGA-NVs were determined using LabChip analyzer and Agilent 2100 bioanalyzer. c, d The presence of CpG-rich genomic DNA sequences and sgRNA targeting Pik3cg was confirmed through PCR amplification and agarose gel electrophoresis. e Subcellular localization of the top 200 abundant proteins, ranked according to their iBAQ protein intensity, was identified in E. coli, protoplasts, OMVs and sgPik3cg-DHP/DGA-NVs through proteomic analysis, with 100 μg of protein used for each sample. A pie chart illustrates the variety of protein types categorized by their subcellular localization in each sample. f Analysis of the Clusters of Orthologous Groups (COG) was conducted for the top 200 abundant proteins identified in sgPik3cg-DHP/DGA-NVs. g The presence of Cas9 protein in E. coli and sgPik3cg-DHP/DGA-NVs was determined using western blotting. All experiments for (a–g) were independently repeated three times, yielding consistent results. Source data are provided as a Source Data file.

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