Fig. 2: Tyrosine residues play a dominant role in mSWI/SNF PLD phase separation.

a A bubble chart representation of the sequence composition of FUS^PLD, FUS^2XPLD, and FUS^{2XPLD half YtoS}. The lengths of the PLDs are displayed as amino acid count “aa”. Fluorescence microscopy images of HEK293T cells expressing GFP-tagged PLDs and variants of (b) FUS^PLD (FUS^PLD, FUS^2XPLD, or FUS^{2XPLD half YtoS}), (c) ARID1A^PLD (ARID1A^PLD, ARID1A^{PLD YtoS} and ARID1A^{PLD 30QtoG}), and (d) BRG1^PLD (BRG1^PLD, BRG1^{PLD Aro+} and BRG1^{PLD Aro++}) as indicated. Hoechst was used to stain the cell nucleus, which is shown in blue. The phase separation capacity is quantified over various levels of nuclear protein concentrations. A phase separation (PS) score of ‘1’ indicates the presence of nuclear condensates and a PS score of ‘0’ represents diffused expression patterns. The shaded regions represent the transition concentrations. Asterisk ‘*’ denotes cytoplasmic concentration. (FUS^PLD n = 25 cells, FUS^2XPLD n = 51 cells, FUS^{2XPLD half YtoS} n = 32 cells, ARID1A^PLD n = 43 cells, ARID1A^{PLD YtoS} n = 29 cells, ARID1A^{PLD 30QtoG} n = 49 cells, BRG1^PLD n = 34 cells, BRG1^{PLD Aro+} n = 38 cells, and BRG1^{PLD Aro++} n = 28 cells from two biological replicates). Also see Fig. S3.