Fig. 4: Tyrosine residues are important for heterotypic interactions within mSWI/SNF PLD condensates. | Nature Communications

Fig. 4: Tyrosine residues are important for heterotypic interactions within mSWI/SNF PLD condensates.

From: Heterotypic interactions can drive selective co-condensation of prion-like low-complexity domains of FET proteins and mammalian SWI/SNF complex

Fig. 4

a HEK293T cells co-expressing GFP-tagged ARID1APLD YtoS and mCherry-tagged OptoFUSPLD constructs. The degree of colocalization is displayed as intensity profiles for condensates shown in the inset images. Green represents the intensity profile of the GFP-tagged construct and red represents the profile for mCherry-tagged construct. b HEK293T cells co-expressing PLDs (BRG1PLD, BRG1PLD Aro+, BRG1PLD Aro++) and mCherry-tagged OptoFUSPLD construct. The degree of colocalization is displayed as intensity profiles for condensates shown in the inset images. Green represents the intensity profile of the GFP-tagged construct and red represents the profile for mCherry-tagged construct. The yellow line indicates the nuclear periphery. c Enrichment coefficients of GFP-tagged BRG1 PLDs within mCherry-tagged OptoFUSPLD condensates. Enrichment is calculated as the ratio of mean intensities from the dense phase and the dilute phase (BRG1PLD n = 89 condensates, BRG1PLD Aro+ n = 106 condensates, BRG1PLD Aro++ n = 81 condensates from two biological replicates). Significance was calculated by a student’s two-tailed t test.

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