Fig. 7: Several secondary transcriptional regulators are activated to regulate developmental stage transition after depletion of AP2XI-2.
From: The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony
a Confocal images showing the expression of secondary transcriptional regulators during merogony after depletion of AP2XI-2 under alkaline conditions for 3 days. The secondary transcriptional AP2 factors shown in blue and green colors on the left side of the figure denote those mainly expressed in mature merozoites and schizonts, respectively. Factors shown in black color denote those with slight increase upon depletion of AP2XI-2. Magenta, anti-IMC1; red, anti-Ty. Scale bar, 10 µm. b Representative images of the indicated parasite strains grown in an alkaline medium for 3 days for induction of merozoites. Magenta, anti-GAP45; red, anti-IMC1. Scale bar, 10 µm. c Quantification of mature merozoites in the indicated strains following 3 days of exposure to alkaline stress. Data represents the mean ± SD from three independent experiments, analyzed by two-tailed, unpaired t test, NS, no significant. d AP2X-3-mAID-6HA was undetected in tachyzoites (32 h post-infection) and bradyzoites (3 days post-infection). Magenta, anti-IMC1; green, FITC-DBL; red, anti-HA. Scale bar, 10 µm. e Representative images of the plaques formed by AP2X-3-mAID-6HA parasites grown in HFF monolayers for 8 days with or without IAA.
