Fig. 3: Film hydrogels exhibited in vitro antibacterial activity and cytocompatibility.

a–d Antibacterial efficacy of PPN(C4) film hydrogels assessed via contact testing for 1 h. # denotes that no bacterial colonies were observed on the agar plate (n = 3 biologically independent samples, two-tailed Student’s t test). e Cell viability of human dermal fibroblasts (HDFs) after incubation with the extracts (blue) or direct contact immersion (red) of PPN(C4) film hydrogels at 37 °C for 24 h (n = 3 cells examined over 3 independent experiments). Data are presented as mean values ± SD.