Fig. 4: Ex vivo human skin equivalent (HSE) model of the film hydrogels. | Nature Communications

Fig. 4: Ex vivo human skin equivalent (HSE) model of the film hydrogels.

From: Hydrogel dressings with intrinsic antibiofilm and antioxidative dual functionalities accelerate infected diabetic wound healing

Fig. 4

a Representative images of MTT staining for wound healing. b Quantitative measurement of the MTT assay at the day 4 and day 7 time points (triplicated; n = 6–9 biologically independent samples, two-tailed Student’s t test, data are presented as mean values ± SEM). c Representative immunostaining images of H&E, p63, Keratin 10 (K10), and Keratin 14 (K14) after treatment with the PPN(C4)−1 hydrogel and its variants (PP-N is without PIM(C4)−1, and PPN- is without NAC). The samples were seriallly sectioned from the same embedded sample and each sample slice with section thickness = 4 μm was used only for one type of staining. Scale bar = 100 μm.

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