Fig. 6: Wound histology of diabetic mouse model treated 24 h post-infection of MRSA USA300 with hydrogel films.

a Representative images depicting (i) H&E and (ii) picrosirius red stained tissue sections of untreated control, silver dressing, PPcontrol, and PPN(C4)−1 hydrogel-treated wounds on day 7 post-treatment. Scale bar = 1 mm. b Quantification of granulation tissue formation thickness based on histological wound samples harvested on day 7 post-treatment. Quantification of (c) collagen III (immature collagen, stained green) and (d) collagen I (native collagen, stained red) deposition and remodeling in the histological wound samples harvested on day 7 post-treatment. (n = 6 mice, two-tailed Student’s t test, data are presented as mean values ± SD).