Fig. 4: Selection and characterization of anti-IL-2Rβ and anti-IL-2Rγ clones.
From: i-shaped antibody engineering enables conformational tuning of biotherapeutic receptor agonists
a Schematic depicting the selection and screening process for binders to IL-2Rβ and IL-2Rγ (created with BioRender.com). b Plots comparing affinities determined by SPR and cell surface binding propensity for 61 anti-IL-2Rβ (top) and 34 anti-IL-2Rγ clones (bottom). Clones that block IL-2 signaling in the Jurkatβγ-STAT5-Luc reporter assay are shown as squares and non-blocking clones are shown as circles. Red and blue symbols indicate selected lead clones for IL-2Rβ and IL-2Rγ, respectively. All other nonlead clones are black. Source data are provided as a Source Data file. c Epitope mapping of lead anti-IL-2Rβ (red box) and anti-IL-2Rγ (blue box) clones. Epitopes on IL-2Rβ and IL-2Rγ are shown in red and blue, respectively. For reference, the IL-2 binding site is highlighted based on the ternary complex (black box, PDB: 2ERJ). In each image, the IL-2Rβ and IL-2Rγ structures are shown on the right and left, respectively. A 90° rotated image of the crystal structure is shown to further depict epitope residues.
