Fig. 3: Assaying growth of ERdd fusion strains for estradiol dependence.
From: Engineering stringent genetic biocontainment of yeast with a protein stability switch
a Spot assay of strains with direct ERdd fusion to a single essential gene at different estradiol concentrations on YPD agar, showing strong dependence on estradiol for all tested ERdd strains. b Growth assay in YPD with 1 µM estradiol, showing strains with growth indistinguishable from that of the parental strain BY4742 in solid lines and ones with a slight apparent growth defect with dashed lines. c All tested ERdd strains show a substantial to very strong growth defect without estradiol. For liquid growth assays means with standard deviations from three independent experimental repeats are shown. Source data are provided as a Source Data file.
