Fig. 2: Exogenous cyclic loading-mediated regulation of osteocytes, osteoblasts, and osteoclasts is abolished in T2D mice. | Nature Communications

Fig. 2: Exogenous cyclic loading-mediated regulation of osteocytes, osteoblasts, and osteoclasts is abolished in T2D mice.

From: Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics

Fig. 2

a Ploton silver staining showing the morphology of the osteocyte canalicular network in male diabetic and non-diabetic tibiae following cyclic compressive loading, and the corresponding statistical result. b–e Immunohistochemical staining of Caspase-3, RANKL and OPG, sclerostin expression in osteocytes (brown) in diabetic and non-diabetic tibial cortical bone. f TRAP staining to label osteoclasts (claret-red) in diabetic and non-diabetic tibial trabecular bone, and the corresponding statistical result of the number of osteoclast per millimeter of bone surface (N.OC/BS). g Toluidine blue staining to label osteoblasts on in diabetic and non-diabetic trabecular bone surface, and the corresponding statistical result of the number of osteoblast per millimeter of bone surface (N.OB/BS). h–j Calcein and alizarin red double labeling of the trabecular, periosteal, and endocortical bone surfaces in diabetic and non-diabetic tibiae, and the corresponding statistical results of mineral apposition rate (MAR). Graphs represent mean ± SD (n = 8 mice per group). *P < 0.05, **P < 0.01 and ***P < 0.001 by two-way ANOVA with Bonferroni’s post test. Specific P values are provided in the Source Data file. Scale bars: a–e, g 20 μm; f, h–j 50 μm.

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