Fig. 5: Microscopy montage of a microfluidics semi-lethal pulse.
From: Plasmid-mediated phenotypic noise leads to transient antibiotic resistance in bacteria
A Montage of a representative time-lapse images of MG:GT growing in a microfluidic device exposed to a semi-lethal pulse of AMP. We obtained data from 5810 lineages from 46 microfluidic chambers. B Overlay time-lapse movie showing the fluorescent intensities of GFP (green) and rhodamine (magenta) in the MG:GT population. C Fraction of the MG:GT population in each cellular state as a function of time (normal cells in blue, stressed in yellow, and dead in orange). Most surviving cells exhibit conditional filamentation upon antibiotic exposure and resume normal growth once the drug is withdrawn. D Fraction of the MG/pBGT population in each cell state. In this case, a smaller fraction of cells produce filaments, as high PCN cells maintain low periplasmic levels of antibiotics and survive without triggering the stress response system. E Overlay of selected frames from the time-lapse movie shows variable GFP expression levels within the population, as well as varying response to the drug exposure. F DIC images of selected frames of the MG/pBGT population growing in a microchemostat. We obtained data from 1077 lineages from 8 separate microfluidic chambers.
