Fig. 4: Seed storage protein in the F₃ seed of XB population.

a Transgressive segregation in seed protein content (%) observed in the XB population (n = 161). b Bar plot showing progressive seed protein accumulation in the maturing seeds of FP15 maternal line, each with biological triplicate replicates (n = 3). Data are presented as mean ± SEM. Different letters indicate statistically significant differences at p < 0.01 by one-way ANOVA test with Tukey post-hoc test (Day 30 vs Day 45, p = 0.0037). c Median values of seed protein content in XB F₃ population (n = 161) were relatively higher (37.0% with few outliers observed) in the heterozygous lines with SNP Marker_33026288 carrying a G/T SNP variation at Chr03.1: 23,735,683 than the GG (35.2%) and TT (36.7%) homozygotes. Box plot (drawn using R geom_boxplot) with the centre line indicates the means, the edges represent 25th and 75th percentile, the whiskers extend to indicate the 1.5 interquartile range from the edges. Different letters indicate statistically significant differences at p < 0.001 by one-way ANOVA test with Tukey post-hoc test (GT vs GG, p = 0.0007). Source data are provided in a Source data file.