Fig. 2: Neutralizing activity, affinity, and antibody effector functions analyses of BA7535 and BA7208. | Nature Communications

Fig. 2: Neutralizing activity, affinity, and antibody effector functions analyses of BA7535 and BA7208.

From: Identification of a highly conserved neutralizing epitope within the RBD region of diverse SARS-CoV-2 variants

Fig. 2

a, b Neutralization curves against the authentic SARS-CoV-2 variants (BA.1, BA.2, BA.5, BF.7, XBB.1, XBB.1.5, XBB.1.9.1 and EG.5) are shown for each of the indicated antibodies. Three biological replicates were performed. ND not detected. The average ± SD from two independent experiments with technical duplicates is shown. c KD values of BA7535 and BA7208 to BA.1 RBD examined by SPR. Data were collected from two biological replicates and represented as Mean ± SD. d–h ADCC and ADCP of BA7535, BA7208, and LY-CoV1404. Experiments were performed in duplicate. Three biological replicates were performed. Data were presented as Mean ± SEM. d ADCC activity of mAbs with CHO-K1 cells expressing SARS-CoV-2 spike (reference strain) as target cells. e ADCP activity of mAbs with CHO-K1 cells expressing SARS-CoV-2 spike (reference strain) as target cells. f Cell binding ability of mAbs against CHO-K1 cells expressing SARS-CoV-2 spike (reference strain). g ADCC activity of mAbs with HEK293T cells expressing SARS-CoV-2 BA.1 spike as target cells. h ADCP activity of mAbs with HEK293T cells expressing SARS-CoV-2 BA.1 spike as target cells. Irrelevant mAb with the same constant region was used as an isotype. Source data are provided as a Source Data file.

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