Fig. 3: T cell CaMK4 drives germinal center formation during immunization. | Nature Communications

Fig. 3: T cell CaMK4 drives germinal center formation during immunization.

From: CaMK4 controls follicular helper T cell expansion and function during normal and autoimmune T-dependent B cell responses

Fig. 3

Camk4fl/fl (red) and Camk4fl/fl.dlck-Cre (blue) mice were immunized with sheep red blood cells and subsequently sacrificed (each point represents an individual mouse). a Gating strategy (left panel) and cumulative data (right panel) of splenic germinal center B cells. b Absolute number of splenic germinal center B cells. c Immunofluorescence of the spleen 7 days after immunization showing T cell (CD3, green), B cells (B220, red), and germinal centers (peanut antigen PNA, blue). d The area of germinal centers (PNA staining) was measured in the spleen of Camk4fl/fl and Camk4fl/fl.dlck-Cre (n = 3 mice per group). Each dot represents the area of one germinal center (AU arbitrary unit). One-way ANOVA with Holm-Sidak’s correction (a, b) or the two-tailed non-parametric Kolmogorov–Smirnov test (d). Bars indicate mean ± s.e.m.

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