Fig. 6: Malignant T cells secret IL-32β isoform to promote classical MHC-I expression on malignant T cells. | Nature Communications

Fig. 6: Malignant T cells secret IL-32β isoform to promote classical MHC-I expression on malignant T cells.

From: MHC-I upregulation safeguards neoplastic T cells in the skin against NK cell-mediated eradication in mycosis fungoides

Fig. 6

a–c Analyzing cytokine-related genes (N = 526 cells for Main Clone (MC); N = 141 cells for Relate Main Clone (RMC); N = 50 cells for Bystander Groups (BG); N = 108 cells for Single Bystanders (SB); N = 85 cells for Healthy (H)). a Malignant T-cell populations show significantly increased IL32 expression and decreased IL7R expression. b IL32β is the predominant IL32 isoform expressed in malignant T‑cell populations. c MHC-I expression positively correlated with IL32 expression, specifically in malignant T-cell populations but not in single bystander T-cell or healthy T-cell populations. The p values were calculated using correlation test. d NanoString analysis showed that elevated levels of MHC-I molecules and IL32 are positively correlated with the proportion of TCR clonal T cells in the micro-environment of MF skin lesions (N = 3) but not in benign inflammatory skin disease (N = 3). The color scale indicates higher gene expression in red and lower gene expression in blue. e IL-32 upregulated at the protein level in T cells from MF skin lesions (N = 3). Data were presented as mean values +/– SEM. The p values were calculated using unpaired, two-tailed student’s t test. siIL32 RNA transfection into My-La CD4+ cells. f RNA interference knockdown of IL32 resulted in decreased expression of IL-32 and three classical MHC-I molecules (HLA‑A, HLA-B and HLA-C) in My-La CD4+ cells (N = 3), analyzed by qRT-PCR. Data were presented as mean values +/– SEM. The p values were calculated using unpaired, two-tailed student’s t test. g siIL32 decreased the viability of My-La CD4+ cells (N = 3). Control siRNA served as a negative control. Data were presented as mean values +/– SEM. The p values were calculated using unpaired, two-tailed student’s t test. h T cells from MF skin lesions show high expression of IL-32β binding sites (IL-32βBS) on their cell surface (N = 3). Data were presented as mean values +/– SEM. The p values were calculated using unpaired, two-tailed student’s t test. i, Increased classical MHC-I expression exclusively on T cells from MF skin lesions in response to IL-32β stimulation (N = 3). The p values were calculated using paired, two-tailed student’s t test. Source data are provided as a Source Data file.

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