Fig. 2: Analytical workflow designed to address the severity of a cohort of patients affected by Congenital Myasthenic Syndromes (CMS).

A multi-scale functional analysis approach, based on multilayer networks, was used to identify the functional relationships between genetic alterations obtained from omics data (Whole Genome Sequencing, WGS; RNA-sequencing, RNAseq) with known CMS causal genes. In green, compound heterozygous variants; in yellow, copy number variants (CNVs); in purple, known CMS causal genes. Modules of CMS linked genes are detected using graph community detection at a resolution range (γ) (Methods) where the most prominent changes in community structure occur. Modules that emerged from this analysis were characterized at single individual level.