Fig. 2: Infection rates and viral loads in mosquito midguts of nine Aedes aegypti populations from Martinique examined 21 days after exposure to an infectious blood meal containing one YFV strain (Bolivia, Ghana, Nigeria, Sudan or Uganda).
From: Evaluating vector competence for Yellow fever in the Caribbean
Mosquitoes were exposed to an infectious blood meal at a titer of 107 FFU/mL using an Hemotek system maintained at 37 °C. Engorged mosquitoes were kept for 21 days in controlled conditions and then dissected to isolate the midgut for estimating the viral load by titration. A–E The infection rate was defined as the proportion of mosquitoes with an infected midgut among the total number of mosquitoes exposed to the blood meal. The error bars correspond to the confidence intervals (95%) for IR (Table S3); **0.001 ≤ p < 0.01 (p = 0.005 for B, p = 0.001 for E), ****p < 0.0001 (A, C, D) by Fisher’s exact test (two-sided). F–J The number of viral particles in individual mosquito midguts (scatter plot) and mean (bar) are shown (Table S3); **0.001 ≤ p < 0.01 (p = 0.0011 for G), ***0.0001 ≤ p < 0.001 (p = 0.0002 for F and p = 0.0001 for H–J) by Kruskall-Wallis non-parametric test (one-sided). In brackets are the numbers of mosquitoes tested. Source data are provided as a Source Data file.
