Fig. 3: Stepwise dissemination rates and viral loads in mosquito carcasses of nine Aedes aegypti populations from Martinique examined 21 days after exposure to an infectious blood meal containing one YFV strain (Bolivia, Ghana, Nigeria, Sudan or Uganda).
From: Evaluating vector competence for Yellow fever in the Caribbean
Mosquitoes were exposed to an infectious blood meal at a titer of 107 FFU/mL using an Hemotek system maintained at 37 °C. Engorged mosquitoes were kept for 21 days in controlled conditions and then dissected to isolate the carcass for estimating the viral load by titration. A–E The stepwise dissemination rate was defined as the proportion of mosquitoes with an infected carcass among mosquitoes with an infected midgut. The error bars correspond to the confidence intervals (95%) for SDR (Table S3); *0.01 ≤ p < 0.05 (p = 0.018 for B), **0.001 ≤ p < 0.01 (p = 0.004 for E), ****p < 0.0001 (C, D) for by Fisher’s exact test (two-sided). F–J The number of viral particles in individual carcasses (scatter plot) and mean (bar) are shown (Table S3); ***0.0001 ≤ p < 0.001 (p = 0.0001 for F, p = 0.0001 for H–J) by Kruskall-Wallis non-parametric test (one-sided). In brackets are the numbers of mosquitoes tested. Source data are provided as a Source Data file.
