Fig. 4: Stepwise transmission rates and viral loads in mosquito saliva of nine Aedes aegypti populations from Martinique examined 21 days after exposure to an infectious blood meal containing one YFV strain (Bolivia, Ghana, Nigeria, Sudan or Uganda).
From: Evaluating vector competence for Yellow fever in the Caribbean
Mosquitoes were exposed to an infectious blood meal at a titer of 107 FFU/mL using an Hemotek system maintained at 37 °C. Engorged mosquitoes were kept for 21 days in controlled conditions and then prepared for salivation to estimate the viral load by titration. A–E The stepwise transmission rate was defined as the proportion of mosquitoes with infectious saliva among mosquitoes with an infected carcass. The error bars correspond to the confidence intervals (95%) for STR (Table S3). F–J The number of viral particles in individual saliva (scatter plot) and mean (bar) are shown (Table S3); *0.01 ≤ p < 0.05 (p = 0.048 for H) by Kruskall-Wallis non-parametric test (one-sided). In brackets are the numbers of mosquitoes tested. Source data are provided as a Source Data file.
