Fig. 5: Number of virus particles (in Log₁₀) detected in the midgut of nine Aedes aegypti populations from Martinique according to mosquito status: with/without dissemination and with/without transmission.

Mosquitoes were examined 21 days after an infectious meal containing one YFV strain (Bolivia, Ghana, Nigeria, Sudan, and Uganda) provided at 10⁷ FFU/mL. Legs and wings of individual mosquitoes were removed, and the proboscis was inserted into a pipette tip to collect saliva. Then, mosquitoes were dissected to isolate the midgut from the carcass. The number of virus particles in midgut, carcass, and saliva were estimated by titration. A–E The number of viral particles in individual midguts (scatter plot) and mean (bar) were estimated for mosquitoes able to disseminate the virus (detection of virus in carcass) versus mosquitoes unable to disseminate the virus (no detection of virus in carcass); ***0.0001 ≤ p < 0.001 (p = 0.0001 for A–E) by Kruskall-Wallis non-parametric test (one-sided). F–J The number of viral particles in individual midguts and mean (bar) were estimated for mosquitoes able to transmit the virus (detection of virus in saliva) versus mosquitoes unable to transmit the virus (no detection of virus in saliva); **0.001 ≤ p < 0.01 (p = 0.0044 for I, p = 0.0077 for J), ***p < 0.001 (p = 0.0001 for F, H) by Kruskall-Wallis non-parametric test (one-sided). In brackets are the numbers of mosquitoes tested. Source data are provided as a Source Data file.