Fig. 4: STAT3 is essential for tumor growth and metastasis in SMARCB1 KO  BLCA.

A Immunoblot analysis showing the confirmation of STAT3 knockdown (shSTAT3) in T24 SMARCB1 KO cell lines (clone 16). Lane 1- T24 ctrl; lane 2- SMARCB1 KO; lane 3- KO with shCtrl; and lane 4- KO with shSTAT3. Lane 3 and 4 cell lines were used for the in vivo experiments. GAPDH was used as loading control. B Top panel shows representative BLI in orthotopic mouse xenograft models showing decreased BLI signal indicating decreased tumor growth in SMARCB1 KO with shSTAT3. Bottom panel shows the scatter plot representing BLI signal on day 25 from xenografts derived from SMARCB1 KO with shCtrl (n = 10) and SMARCB1 KO with shSTAT3 (n = 13) [Data are represented as mean ± standard deviation (SD)]. C Scatter plot represents the weight of orthotopic mouse bladders harboring tumors (endpoint; day 27) from SMARCB1 KO + shCtrl (n = 10) and SMARCB1 KO + shSTAT3 (n = 13) [Data are represented as mean ± standard deviation (SD)]. D Left panel shows the representative ex-vivo BLI signal of lungs from mice bearing T24 SMARCB1 KO with scrambled sh or STAT3 KD in SMARCB1 KO orthotopic xenografts. Right panel shows the scatter plot representing the quantification of BLI signal of lungs from SMARCB1 KO with shCtrl (n = 10) and SMARCB1 KO with shSTAT3 (n = 13) [Data are represented as mean ± standard deviation (SD)]. For panels B, C and D, P-values were determined by unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.