Fig. 3: Effect of local CB1 receptor blockade on gamma oscillations and spontaneous calcium events in the anterior piriform cortex.

a Schematic of the implanted device: a tungsten wire electrode is attached to a local injection cannula for LFP monitoring in the vicinity of local drug infusion in freely moving mice. Adaptation from Paxinos and Watson mouse brain atlas. b Mean change in power spectrum induced by Vehicle (black) or AM251 (orange) local injections. Means, bold lines; SEM, shaded areas. Two-way ANOVA interaction: ***P < 0.001. Horizontal line indicates post-hoc t tests with Bonferroni correction: *P < 0.05. n = 14 sessions from 8 mice for both Vehicle and AM251. c Schematic of the experimental approach: fiber photometry system to record anterior piriform cortex (aPC) excitatory cells calcium signal (GCaMP6f is expressed under CamKII promoter) following local drug delivery in vivo. Adaptation from Paxinos and Watson mouse brain atlas. d Histological sagittal section showing the recording/injection site. Blue, DAPI; green, GCaMP6f; red, autofluorescence with position of injection cannula. Similar histological verifications were conducted in all recorded mice. e Example of spontaneous calcium events monitored following local injection of Vehicle and AM251. f Median occurrence rate of spontaneous calcium events. Vehicle 0.33 [0.27 0.40] events/s vs AM251 0.23 [0.18 0.29] events/s. Two-sided Mann–Whitney test: **P = 0.002; n = 16 and 20 hemispheres from 8 and 10 mice for Vehicle and AM251, respectively. Boxplots and values in the legend represent median and 25–75th percentiles ([lower bound upper bound]). Source data are provided as a Source Data file.