Fig. 2: Analysis of nucleus-cytoplasm volumetric coupling across cell division reveals general relations linking nucleus and cytoplasm volumes.
a Experimental detection example of I (NEB), II (roundup onset) and III (PME). b Representative experimental images of an RPE1 cell across division. From top to bottom: transmission light, H2b-BFP, GFP-NES and Texas red channels. Scale bar is 20 µm. c Cumulative distribution of delay between NEB and PME for all the 5 cell lines analyzed. d Boxplot representing the distribution of the volumetric ratio between NEB and PME for the nucleus and cytoplasm. e Boxplot of the NC ratios at NEB and PME. NC ratio increases by 32%, 22%, 20%, 26%, and 23% for the 5 cell lines, respectively. P value of corresponding two tailed paired t test: 2.2·e−16, 1.0·e−10, 3.4·e−8, 5.5·e−9, 0.0001. Rpe1 cytoplasm (f) and nucleus (g) specific volume growth rate, defined as the binned average of \(\frac{1}{V}\cdot \frac{{dV}}{{dt}}\) at fixed volume V, plotted as a function of volume (mean ± SE). In (c), (d) and (e): RPE1 n = 82, MCF10-A n = 88, DCIS.com n = 66, MCF10-CA n = 66, MCF7 n = 42. In (f) and (g): Rpe1 n = 99. In (d) and (e) boxplots, middle bars are medians, the rectangles span from the first to the third quartiles and the bars extent from ±1.5*IQR. Source data are provided as a Source Data file. “n” represents the number of cells examined over at least three independent experiments.
