Fig. 3: Analysis of promoter PRO-seq signal reveals that SC selective genes accumulate paused Pol II in SG. | Nature Communications

Fig. 3: Analysis of promoter PRO-seq signal reveals that SC selective genes accumulate paused Pol II in SG.

From: RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis

Fig. 3

a Heatmaps show the relative promoter PRO-seq signal (TSS to +150, left) as compared with the gene body PRO-seq signal (at right, as in Fig. 2e, right). Genes are ranked as in Figs. 1f, 2e. b–d Plots of average relative signal from PRO-seq within promoter (dotted line with triangles) or gene body (dashed line with squares) regions for each cell type. Shown are data for genes in clusters 1 and 2 (b) cluster 6 (c) or cluster 4 (d). e Genome browser view of RNA-seq and PRO-seq at Fnta, a SC-specific cluster 4 gene that has a prominent peak of promoter PRO-seq in SG. f–g Metagene plots of average PRO-seq reads in each cell type for all protein-coding genes in cluster 4 (f) or cluster 5 (g). Inset y-axis is zoomed-in to show gene body signal from +125 to +1500 nt downstream of the TSS. Source data are provided as Source Data Fig. 3.

Back to article page