Fig. 4: NELF-B is required for the successful completion of spermatogenesis. | Nature Communications

Fig. 4: NELF-B is required for the successful completion of spermatogenesis.

From: RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis

Fig. 4

a–d Immunohistochemistry using NELF-B antibody stains NELF-B-positive Sertoli (S) and germ cells (G) in testis from control PND15 (a) and PND24 (c) mice. Quantification confirms 100% positive staining in n = 50 tubules from mice at PND15 and PND24. In tubules from NELF-B cKO mice at PND15 (b) and PND24 (d), the germ cells (G) are devoid of NELF-B protein while the Sertoli (S) cells remain NELF-B positive. Quantification identified n = 10 out of n = 72 tubules (13.89% positive) at PND15, and n = 8 out of n = 50 tubules (16% positive) at PND24. Scale bar, 50 μm. We note that some NELF-B positive germ cells are detected in the NELF-B cKO, which represent cells that escaped Cre-mediated excision of NELF-B. e–h Testis cross sections of PND15 and PND24 control (n = 3, e, g) or NELF-B cKO (n = 3, f, h) mice. PND15 NELF-B cKO mice showed degeneration of the epithelium (asterisk) involving meiotic cells in some areas (f). At PND24, vacuoles are also formed, and the epithelium is disorganized with detached and fewer RS (h). Scale bar, 50 μm.

Back to article page