Fig. 7: Promoter activity in SG correlates with sites of double-strand breaks in SC.

a Metagene plots of SPO11-oligo reads around the TSS for all DE genes (n = 17,078). b Heatmaps of SG PRO-seq (left, dark blue) or SPO11 oligo reads (right, gray), aligned at TSSs (indicated by arrow) with genes ranked by SG promoter PRO-seq signal (TSS to TSS + 150, n = 17,078). c, d Metagene plots around the TSSs for the top (c) or bottom (d) quartile based on SG PRO-seq reads in promoters with at least 5 reads (n = 14,804, with n = 3701 genes per quartile). PRO-seq signal for SG (blue) is plotted on the left y-axis, SPO11 oligo reads (gray) is plotted with the right y-axis. e Box plots of the distribution of SPO11 reads at each quartile (n = 3701) of SG PRO-seq reads at promoters with at least 5 reads (n = 14,804). Line represents the median, whiskers represent 1.5X interquartile range. P-values from two-sided Wilcoxon signed rank test. f Testis cross sections of PND24 control (n = 2, left) or NELF-B cKO (n = 2, right) mice. L/Z, indicates SC in Leptotene/Zygotene stages, and P, indicates pachytene SCs. Scale bar, 50 μm. g Quantification of SC at Leptotene/Zygotene versus Pachytene stages per tubule. Shown are the mean and range of n = 2 biologically independent animals. P-value is from Unpaired two-sided t-test. Source data are provided as Source Data Fig. 7.