Fig. 8: NELF-B cKO mice have impaired DSB repair. | Nature Communications

Fig. 8: NELF-B cKO mice have impaired DSB repair.

From: RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis

Fig. 8

a SC showing SYCP3 (red), ɣ-H2AX (green) and DAPI (blue) in control (top row) and NELF-B cKO (bottom row). ɣ-H2AX signal is restricted to the sex body (red arrow) in control whereas it persists on autosomes (white arrows) in NELF-B cKO indicating impairment of DSB repair. Scale bars = 20 μm. At right: Quantification of relative ɣ-H2AX area (ɣ-H2AX/DAPI) between the control (n = 40) and NELF-B cKO (n = 52) from three biological replicates. Mean indicated with horizontal line. Error bars represent standard error of the mean. P-value from two-sided t-test. b SC showing SYCP3 (green) and RPA (red) in control (top row) and NELF-B cKO (bottom row); the higher number of RPA foci in NELF-B cKO implies impaired DSB repair. Scale bars = 20 μm. At right: Quantification of the RPA foci numbers at pachytene from control (n = 51) and NELF-B cKO (n = 46) from three biological replicates. Mean indicated with horizontal line. Error bars represent standard error of the mean. P-value from two-sided t-test. Source data are provided as Source Data Fig. 8.

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