Fig. 4: Gamma and ancestral RBD formulated with Alum induce T cell responses in mice. | Nature Communications

Fig. 4: Gamma and ancestral RBD formulated with Alum induce T cell responses in mice.

From: A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection

Fig. 4

BALB/c mice were immunized at day 0 and day 14 via i.m. with: Gamma RBD + Alum (blue circles) or Ancestral RBD + Alum (red triangles) and 28 days after T cell responses were evaluated. a Splenocytes were stimulated with complete medium or a peptide pool derived from RBD (Gamma and Ancestral) and then brefeldin A was added. Afterward, cells were harvested and stained with specific Abs anti-CD8, and anti-CD4, fixed, permeabilized, and stained intracellularly with anti–IFN-γ, TNF-α and anti-IL-2. Results are presented as percentage of cytokine-producing T cells. Bars are means ± SEM. n = 6 mice per group. Kruskal Wallis test. *p < 0.05, **p < 0.01, ***p < 0.001. Exact P values are shown. Representative of two experiments. b Evaluation of secreted cytokines in the supernatants from stimulated splenocytes by flow cytometry. Bars are means ± SEM. n = 5 mice per group. Kruskal Wallis test. *p < 0.05, **p < 0.01. Exact P values are shown. Representative of two experiments. Source data are provided as a Source Data file.

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