Fig. 5: Vaccination with Gamma RBD + Alum protects K18-hACE2 transgenic mice against intranasal Omicron BA.5 SARS-CoV-2 infection. | Nature Communications

Fig. 5: Vaccination with Gamma RBD + Alum protects K18-hACE2 transgenic mice against intranasal Omicron BA.5 SARS-CoV-2 infection.

From: A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection

Fig. 5

Mice were i.m. inoculated on days 0 and 14 with: (i) placebo, (ii) Gamma RBD + Alum, (iii) Ancestral RBD + Alum or (iv) bivalent BNT162b2. n = 12 mice per group. Two weeks following immunization, mice were intranasally infected with 1 × 104 PFU of Omicron BA.5. a Viral RNA copies were determined by RT-qPCR in lung homogenates at 3 and 5 dpi (n = 6 mice per time point). Bars are means ± SEM. Kruskal Wallis test. *p < 0.05. Exact P values are shown. b Pathological scores according to the severity of histological parameters were calculated for each group of mice at 5 dpi (n = 6 mice per group). Scores of all parameters were summed (Combined). Bars are means ± SEM. Kruskal Wallis test. *p < 0.05, **p < 0.01. Exact P values are shown. c Hematoxylin and eosin (H&E) staining of the lung sections at 5 dpi.Placebo (A), vaccinated mice (B–D) and an uninfected animal (E, naive) are shown. Scale bars, 50 µm. Representative of two experiments. Source data are provided as a Source Data file.

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