Fig. 4: Rab13 is recruited to Shigella BCV for membrane translocation.

A Time-lapse microscopic images of the recruitment of Rab13 (gray) in transfected HeLa cells. Galectin-3-mOrange (red) was used as a marker of vacuolar rupture. Images were captured every minute and maximum z-projection of the representative infection focus is shown. Blue arrowheads indicate the localization of Rab13-positive BCV while the magenta arrowheads indicate some Rab13-positive infection-associated macropinosome. Scale bars are 5 μm. B Analysis of BCV membrane pulling or fragmentation as illustrated in Fig. 1B, C in cells expressing Rab13 and the GDP-mimicking mutant Rab13-T22N. The bars represent mean ± SEM of at least three independent replicates. C Analysis of the fates of individual Shigella BCVs in cells expressing Rab13 or Rab13-T22N. The bars represent mean ± SEM of three independent replicates. Analysis of the D time of Shigella BCV disassembly and E Shigella escape time in cells expressing Rab13 or Rab13-T22N. n > 50 infected cells in three independent replicates. The data are presented as mean(magenta bars) ± SEM and two-tailed Welch’s t-tests were performed (**p < 0.01). Source data are provided as a Source Data file.