Fig. 8: C15ORF48 is required for prevention of autoimmunity in mice.
a Immunostaining of tissue sections from Rag1–/– mice (C57BL/6 background, 8–10-week-old) with sera from 21-week-old wild-type and C15orf48–/– mice (C57BL/6 background). Nuclei were counter-stained with DAPI. Each box represents serum from a single mouse (wild-type, n = 5 mice; C15orf48–/–, n = 7 mice). The gray box indicates the detection of reactivity in tissue sections from Rag1–/– mice. b Western blotting of tissue lysates from Rag1–/– mice with sera from 21-week-old wild-type and C15orf48–/– littermate mice. All sera were diluted equally at 1:1000 with 5% skim-milk containing tris buffer. Western blotting using these sera was performed simultaneously to enable exact comparison of their immunoreactivities. Anti-actin blots were used as loading controls. Data are representative of five independent experiments with sera from five different pairs of littermate mice. c Hematoxylin and eosin staining of lung, kidney, and liver sections from 21-week-old wild-type and C15orf48–/– mice. Inflammatory cell infiltrations are indicated by arrows (left). Infiltration scores were determined based on hematoxylin and eosin staining scored from 0 to 4, in a double-blind manner. Each point represents a value from an individual mouse. Statistical significance was calculated using two-tailed unpaired Student’s t test (wild-type, n = 6 mice; C15orf48–/–, n = 7 mice) (right). d Immunostaining of kidney sections from 21-week-old wild-type and C15orf48–/– mice with anti-mouse IgG. Nuclei were counter-stained with DAPI. Glomerulus-like cell clusters are encircled by white lines. (left). IgG deposition scores were determined based on IgG fluorescence intensities scored from 0 to 4, in a double-blind manner. Each point represents a value from an individual mouse. Statistical significance was calculated using two-tailed unpaired Student’s t test (wild-type and C15orf48–/–, n = 4 mice) (right).
