Fig. 9: C15ORF48 deficiency in thymic stroma causes autoimmunity. | Nature Communications

Fig. 9: C15ORF48 deficiency in thymic stroma causes autoimmunity.

From: Mitochondrial protein C15ORF48 is a stress-independent inducer of autophagy that regulates oxidative stress and autoimmunity

Fig. 9

a A schematic representation of thymic stroma transplantation experiments (left). A representative image of thymus re-generated into a renal capsule of a nude mouse eight weeks after the transplantation (right). b Immunostaining of tissue sections from Rag1–/– mice with sera from WT/nu and KO/nu mice eight weeks after the transplantation. Nuclei were counter-stained with DAPI. Each box represents serum from a single mouse (WT/nu and KO/nu, n = 5 mice). The gray box indicates the detection of reactivity in tissue sections from Rag1–/– mice. c Hematoxylin and eosin staining of tissue sections from WT/nu and KO/nu mice eight weeks after the transplantation. Inflammatory cell infiltrations are indicated by arrows (top). Infiltration scores were determined based on hematoxylin and eosin staining scored from 0 to 4, in a double-blind manner. Each point represents a value from an individual mouse. Statistical significance was calculated using two-tailed unpaired Student’s t test (WT/nu and KO/nu, n = 5 mice) (bottom).

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