Fig. 3: Depletion of neutrophils protects PRL2 myeloid cell deficient mice from severe malaria related acute lung injury.

a A schematic showing the experimental design for neutrophil depletion in P. berghei ANKA (PbA) infection model. PbA infected wildtype (WT) and PRL2 myeloid cell conditional knockout (CKO) mice were intraperitoneally injected with anti-Ly6G monoclonal antibody or an isotype control at 6 days post infection (dpi). b Number of neutrophils in peripheral blood from the four groups of mice described in (a) at 7 dpi (n = 8 mice per group). c Survival curve of the mice described in (b). d Hematoxylin-eosin (H&E) staining of histologic lung sections from the four groups of mice described in (a) at 7 dpi (n = 6 mice per group). Representative images are shown. Scale bars, left: 50 μm, right: 10 μm. e Pulmonary pathology scores and infiltrated neutrophils numbers were quantified from (d). f Quantification of neutrophils and neutrophil extracellular traps (NETs) in immunofluorescence images of lungs from the four groups of mice described in (d) at 7 dpi. g Representative immunofluorescence images of lungs as described in (f). DNA is stained in blue (DAPI), myeloperoxidase is stained in green (MPO) and citrullinated histone H3 is stained in red (Cit-H3). Scale bars, 20 μm. Neutrophils are indicated as co-stained with MPO and DAPI. NETs are indicated as co-stained with MPO and Cit-H3. All data were pooled from two independent experiments. Data are presented as the mean ± SEM or in violin plots showing the median and interquartile range. p values were calculated by two-way ANOVA with Tukey’s multiple testing (b, e, f) or log-rank test (c) and shown in the figures. Source data are provided as a Source Data file.