Fig. 4: PRL2 deficiency promotes NET formation. | Nature Communications

Fig. 4: PRL2 deficiency promotes NET formation.

From: PRL2 regulates neutrophil extracellular trap formation which contributes to severe malaria and acute lung injury

Fig. 4

a Representative immunofluorescence images of neutrophil extracellular traps (NETs) released by wildtype (WT) and PRL2 knockout (KO) bone marrow derived neutrophils (BMNs) after phorbol myristate acetate (PMA), iRBC and hemin stimulation. Fixed cells were stained. DNA is stained in blue (DAPI), and extracellular DNA is stained in green (Sytox Green, SG). Scale bar, 5 μm. DIC, differential interference contrast. Arrowheads indicate NETs. b Quantification of NETs in (a). Data were pooled from three independent experiments. BMNs were collected from different mice (n = 3 per group). c Equivalent amount of WT (in white) and PRL2 deficient TdTomato+ (in red) neutrophils were mixed and then stimulated with PMA together for 5 h. Extracellular DNA is stained in green (SG). Quantitative analysis of NETosis/30 min in each field by live cell imaging. Data were pooled from three independent experiments. BMNs were collected from different mice (n = 3 per group). d Representative time-lapse images from live cell imaging described in (c) are shown. Black/white boxes indicate NETs from WT/KO BMNs, respectively. Scale bars, 25 μm. Violin plots show the median and interquartile range. p values were calculated by two-way ANOVA with Tukey’s multiple testing (b) or two-tailed unpaired t test (c) and shown in the figures. Source data are provided as a Source Data file.

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