Table 1 Successful virus isolation and titration from positive quantitative Reverse-Transcription PCR samples collected from adult filovirus-inoculated AFBs

From: Selective replication and vertical transmission of Ebola virus in experimentally infected Angolan free-tailed bats

 

EBOV

MARV

 

5 dpi

10 dpi

5 dpi

10 dpi

TISSUE

PCR;

(VI)

PCR;

(VI)

PCR;

(VI)

PCR;

(VI)

Lymph Node

3/3;

--

2/2;

(0/1)

1/3;

(0/1)

0/2;

--

Uterus/Placenta

1/1;

(1/1)

1/1;

(1/1)

  

1/1;

(1/1)

Blood

3/3;

(1/1)

1/2;

--

    

Spleen

3/3;

(1/2)

2/2;

--

    

Colon

3/3;

(1/1)

1/2;

--

    

Kidneys

3/3;

(1/1)

1/2;

--

    

Bladder

3/3;

(2/2)

1/2;

--

    

Testes

2/2;

(1/1)

0/1;

--

    

Brain

3/3;

(1/1)

0/2;

--

RESTV

Salivary Gland

2/3;

(0/2)

1/2;

--

Oral Swab

3/3;

(1/2)

0/2;

--

5 dpi

10 dpi

Rectal Swab

3/3;

(1/2)

1/2;

--

PCR;

(VI)

PCR;

(VI)

Spleen

3/3;

(1/2)

2/2;

--

2/3;

(0/1)

0/3;

--

Gallbladder

1/1;

(1/1)

2/2;

(1/1)

1/3;

(0/1)

2/3;

--

Stomach

    

2/3;

(0/1)

0/3;

--

Skin Inoculation

    

3/3;

(1/2)

0/3;

--

Pooled faeces*

3 dpi (+/+); 5 dpi (+/−); 10 dpi (+/−)

    

Pooled urine*

10 dpi (+/−)

    
  1. PCR, polymerase chain reaction; VI, Virus isolation; dpi, days post inoculation.
  2. * Only dpi collection with attempted virus isolation shown: (PCR/VI). (+) Positive; (-) Negative; (--) Virus isolation not attempted: virus RNA loads below the cut-off.
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