Fig. 4: Maternal cifAwAlbB(TIV) expression rescues CI induced by paternal cifA/BwAlbB(TIV) expression.
a Construct design of exu-cifA and shu-cifA. b Manipulating the paternal Cif dosage in transgenic males improves the capacity of exu-cifA females to rescue incompatibility. Expression of cifA in females does not affect their fertility when crossed with wild-type (wt) males. c Rescue capacity of transgenic females is increased when cifA expression is regulated by the shu promoter. In both b and c, Lines denote median and error bars interquartile ranges, numbers in parentheses denote the n. Letters indicate significant differences with an α = 0.05 calculated by a Kruskal–Wallis test: (b H = 311.0, P < 0.0001, d.f. = 11)(c H = 243.2, P < 0.0001, d.f. = 7) followed by a two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli to correct for multiple comparisons, individual P-values are listed in Source Data File. d The expression of cifA relative to rps17 was higher in gravid ovaries taken from shu-cifA compared to exu-cifA females (two-tailed Mann–Whitney U test P < 0.0001, mean and s.d are shown, n = 12 for both groups). Source data are provided as a Source Data file.
