Fig. 6: Expression of Ol-RanT27N causes spindle assembly defects followed by abnormal chromosome segregation. | Nature Communications

Fig. 6: Expression of Ol-RanT27N causes spindle assembly defects followed by abnormal chromosome segregation.

From: Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos

Fig. 6

a Schematic representation of Ran-GTP-mediated spindle assembly by activated SAFs. b Live-cell images of 8-cell stage embryos showing normal spindle assembly in control (left) and abnormal spindle formation followed by severe chromosome mis-segregation in Ol-RanT27N-expressing embryos (right). c Graphs of fluorescence intensities for line scans of Ol-RanWT- (top) or Ol-RanT27N (bottom)-expressing metaphase spindles shown in (b). An arrow in (c) indicates the decrease of MT intensity at the spindle midplane. d Scatter plots showing nuclear size in 8-cell or 16 cell stage blastomeres expressing Ol-RanWT, Ol-RanT27N, or Ol-RanQ72L mutants. e Live-cell images of Ol-RanT27N-expressing embryos showing symmetrical (left) or asymmetrical (right) nuclear shape at NEBD (t = 0), which lead to symmetrical or asymmetrical spindle structure (t = 9) and chromosome segregation (t = 12–15), respectively. f Achromosomal mitosis showing no spindle formation between centrosomes. Scale bars = 100 μm. Source data for (c, d) are provided as a Source Data file.

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