Fig. 8: RCC1 protein knockdown diminishes the dense MT network at the spindle center in metaphase. | Nature Communications

Fig. 8: RCC1 protein knockdown diminishes the dense MT network at the spindle center in metaphase.

From: Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos

Fig. 8

a Live-cell images of control (left) and RCC1-depleted (right) 4-cell blastomeres showing disruption of the dense MT network at the spindle midplane in RCC1-depleted cells (an arrow). b Graphs of fluorescence intensities for line scans of mCh-α-tubulin in (a), showing a decrease of mCh-α-tubulin intensity at the spindle midplane in RCC1-knockdown spindles. c, d Quantification of spindle width (c) and length (d) in control and RCC1-KD spindles. e Symmetrical or asymmetrical nuclear position at NEBD (t = 0) correlated with chromosome-bridge (left) and non-disjunction (right) phenotypes, respectively, in RCC1-KD blastomeres. f, g Live-cell images (f) and quantification (g) of mCherry-α-tubulin in control (left) and RCC1-KD blastomeres in the presence of 330-nM nocodazole. Error bars indicate mean ± SD. Scale bars = 100 μm. Source data for (b–d, g) are provided as a Source Data file. Two-sided Welch’s t-tests were performed for (c, d, g). ***p < 0.001 and ****p < 0.0001.

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