Fig. 9: AID2-mediated RCC1 depletion in blastula embryos causes minor defects in spindle assembly and chromosome segregation. | Nature Communications

Fig. 9: AID2-mediated RCC1 depletion in blastula embryos causes minor defects in spindle assembly and chromosome segregation.

From: Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos

Fig. 9

a Live-cell images showing fluorescence of RCC1-mACF and miRFP6700-Nano3-H2B in control (lower) and OsTIR1(F74G)-expressing (upper) embryos. 5-Ph-IAA was added 8 hr post-injection (hpi). b Live-cell images showing fluorescence of RCC1-mACF, mCh-α-tubulin, and miRFP6700-Nano3-H2B in control (left) and OsTIR1(F74G)-expressing (right) embryos. c Scatterplots showing nuclear size in control and RCC1 KD blastomeres in blastula-stage embryos. d Live images of mCh-α-tubulin in control and RCC1-depleted metaphase cells (top). Graphs of fluorescence intensities for line scans of mCh-α-tubulin in (d), showing a decrease of mCh-α-tubulin intensity at the spindle midplane in RCC1-knockdown spindles. e Ratio of spindle length and cell diameter in control (n = 30) and RCC1 knockdown (n = 35) cells. f Relative intensity (Min/Max) of mCh-α-tubulin fluorescence on the spindle in control (n = 15) and RCC1-KD blastomeres (n = 16). g Scatterplots of mitotic duration in control (10.2 ± 1.5, n = 30) and RCC1-depleted (12.3 ± 1.7, n = 35) cells. h Quantification of normal and abnormal anaphase with chromosome bridges in control and RCC1-depleted cells. i Representative live-cell images showing nuclear reformation defects in RCC1 knockdown cells. Error bars indicate mean ± SD. Scale bars = 100 μm (a) and 10 μm (b, d, i). Source data for (c–h) are provided as a Source Data file. Two-sided Welch’s t-tests were performed for (e–g). **p < 0.01 and ****p < 0.0001.

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