Fig. 2: Eosinophils influence steady-state bone remodeling. | Nature Communications

Fig. 2: Eosinophils influence steady-state bone remodeling.

From: Eosinophils preserve bone homeostasis by inhibiting excessive osteoclast formation and activity via eosinophil peroxidase

Fig. 2

a Percentage of eosinophils (Siglec-F+CD11b+Ly6GintCD45+ cells) in the bone marrow (BM) of wild type (WT) and ΔdblGATA mice (n = 17, 11) analyzed by flow cytometry (FC). b, c 3D trabecular structure by micro-computed tomography (µCT) (b) and quantification of bone volume per total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) (c) in the tibial bone from WT and ΔdblGATA mice (n = 11, 19). Scale bar, 1 mm. d, e Representative tartrate-resistant acid phosphatase (TRAP) staining (d) and quantification of osteoclast surface per bone surface (Oc.S/BS), osteoclast number per bone perimeter (Oc.N/B.Pm), and osteoclast number per tissue area (Oc.N/T.Ar) (e) in tibial bone sections from WT and ΔdblGATA mice (n = 11, 19). Triangles highlight polynucleated osteoclasts. Scale bar, 100 µm. f mRNA expression of Tnfrsf11a, Traf6, Nfatc1, Acp5, Ctsk, and Mmp9 in the long bone of WT and ΔdblGATA mice (n = 5, 4). g Percentage of eosinophils in the BM of control 4get and IL-5tg/4get mice (n = 9) analyzed by FC. h, i 3D µCT trabecular structure (h) and quantification of BV/TV, Tb.N, Tb.Th, and Tb.Sp (i) in tibial bone from 4get and IL-5tg/4get mice (n = 11, 13). Scale bar, 1 mm. j, k Representative TRAP staining (j) and quantification of Oc.S/BS, Oc.N/B.Pm, and Oc.N/T.Ar (k) in tibial bone sections from 4get and IL-5tg/4get mice (n = 13, 11). Triangles illustrate polynucleated osteoclasts. Scale bar, 100 µm. l mRNA expression of Tnfrsf11a, Traf6, Nfatc1, Acp5, Ctsk, and Mmp9 in the long bone of 4get and IL-5tg/4get mice (n = 9, 7/8). Data are shown as mean ± SEM. Symbols represent individual mice. P values were determined by two-tailed Mann–Whitney test (2a, 2c BV/TV, 2i, 2l Tnfrsf11a, Mmp9) or unpaired two-tailed t test (2c Tb.N, Tb.Th, Tb.Sp, 2e, 2f, 2g, 2k, 2l Traf6) for single comparisons. Asterisks mark statistically significant difference (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Source data are provided as a Source data file.

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