Fig. 6: Hyper-eosinophilia reduces pathological bone loss. | Nature Communications

Fig. 6: Hyper-eosinophilia reduces pathological bone loss.

From: Eosinophils preserve bone homeostasis by inhibiting excessive osteoclast formation and activity via eosinophil peroxidase

Fig. 6

a Experimental outline of ovariectomy (OVX) in 4get controls vs. IL-5tg/4get mice. b Percentage of eosinophils (Siglec-F+CD11b+Ly6GintCD45+ cells) and osteoclast precursors (OCPs/CSF1R+Ly6ChighCD11b+CD45+ cells) in the BM of 4get sham and OVX mice vs. IL-5tg/4get sham and OVX mice (n = 10, 10, 8, 8). c, d 3D trabecular structure by µCT (c) and quantification of bone volume per total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) (d) in tibial bone from the aforementioned 4 groups (n = 7, 10, 5, 6). Scale bar, 1 mm. e, f Representative TRAP staining (e) and quantification of osteoclast surface per bone surface (Oc.S/BS), osteoclast number per bone perimeter (Oc.N/B.Pm), and osteoclast number per tissue area (Oc.N/T.Ar) (f) in tibial bone sections from the aforementioned 4 groups (n = 7, 10, 5, 6). Triangles illustrate osteoclasts. Scale bar, 100 µm. g mRNA expression of Epx in long bone in the aforementioned 4 groups (n = 10, 10, 8, 8). h Experimental outline of serum transfer arthritis (STA) in 4get controls vs. IL-5tg/4get mice. i Percentage of eosinophils and OCPs in the BM of control and STA-induced 4get mice vs. control and STA-induced IL-5tg/4get mice (n = 5, 5, 6, 6). j, k µCT 3D trabecular structure (j) and quantification of BV/TV, Tb.N, Tb.Th, and Tb.Sp (k) in tibial bone from the aforementioned 4 groups (n = 5, 5, 6, 6). Scale bar, 1 mm. l, m Representative TRAP staining (l) and quantification of Oc.S/BS, Oc.N/B.Pm, and Oc.N/T.Ar (m) in tibial bone sections from the aforementioned 4 groups (n = 5, 5, 6, 6). Triangles illustrate osteoclasts. Scale bar, 100 µm. n mRNA expression of Epx in long bone in the aforementioned 4 groups (n = 5, 5, 6, 6). Data are shown as mean ± SEM. Symbols represent individual mice. P values were determined by Kruskal–Wallis Dunn’s test (6b, 6i BM Eos) or two-way ANOVA Tukey’s test (6d, 6f, 6g, 6i BM OCPs, 6k, 6m, 6n) for multiple comparisons. Asterisks mark statistically significant difference (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Source data are provided as a Source data file.

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