Fig. 1: Aneuploidy promotes the metastasis of teratomas derived from ESCs.

a Schematic overview of the experiment. LUC, luciferase; PBMCs, peripheral blood mononuclear cells; CTC-like cells, circulating tumor cell-like cells isolated from the peripheral blood of aneuploid teratoma-bearing mice; scRNA-seq, single-cell RNA sequencing; WGS, whole-genome sequencing; WES, whole-exome sequencing. b Whole-body BLI imaging of aneuploid teratoma-bearing mice. c Ex vivo BLI imaging of lung metastases from mice bearing aneuploid teratomas. The rainbow gradient bar represents the photon flux. d Images of lung metastases. The yellow dotted lines indicate metastatic lung lesions. e HE analysis of metastatic foci. Scale bars, 100 μm. f Confirmation that metastases were derived from the injected ESCs by genomic PCR. Histologic analysis of (e) and (f) are reproducibility available. Source data are provided as a Source data file. g Timeline of metastatic events observed in mice bearing different aneuploidies. Ts, trisomy. h Summary of aneuploid ESCs-derived teratoma metastasis. Met/total, the number of metastatic mice in the total observed mice. Sites, metastatic sites. L, lung; MAT, mediastinal; LV, liver; KD, kidney; INT, intestine; SP, spleen; STO, stomach. See also Supplementary Tables 1 and 2.