Fig. 4: Characteristics of cultured CTC-like cells.

a Two CTC-like cell lines (1# and 2#) were successfully established from the peripheral blood of Ts6 + 8 teratoma-bearing mice. Scale bars, 50 μm. Micrograph photos of CTC-like cell lines are reproducibility available. b CNV patterns of cultured CTC-like cells (1# and 2#). c Ts6 + 8 CTC-like cells (1# and 2#) exhibited an enhanced invasion ability compared with that of WT ESCs according to Transwell assays. Scale bars, 200 μm; For each group, n = 10. Error bars, ± SD. P values were calculated using a two-tailed t test. Source data are provided as a Source data file. d BLI imaging signals showed the CTC-like cells (1#) colonized multiple sites (n = 5). The rainbow gradient bar represents the photon flux. e Metastatic lung lesions were observed one month after the tail vein injection of CTC-like cells (1#). Blue dotted lines indicate metastatic nodes.