Fig. 5: scRNA-seq analysis identifies stem-like aneuploid cell clusters in metastatic samples.

a UMAP projection of scRNA-seq data. DC, dendritic cells. ILCs, innate lymphoid cells. ES, derived from aneuploid or WT cells. ES_Ori, original ESCs. ES_Stem, stem cell population. ES_NSC, neural stem cells. ES_Oligo, oligodendrocyte. ES_Schw, Schwann cell. ES_NC, neuron cells. ES_FB, fibroblast cells. ES_Musc, Muscle cells. b Expression of EGFP and luciferase in EGFP-luciferase dual-labeled cells and expression of Pou5f1 and Dppa5a in ESCs. c The proportion of stemness (ES_Ori and ES_Stem) is depicted in different samples, including cell lines, primary teratomas, and metastases. d Pseudotime analysis-reconstructed differentiation routes (branches and nodes) of different cell types recognized by scRNA-seq data analysis. e Heatmap plot displaying the expression of specific genes (listed on the right) associated with the proteasome that were consistently decreased among various differentiation stages (from early to late) across the WT and aneuploid cells in pseudotime order (d) (scaled by row). f Differentially expressed genes in ES_Stem compared with ES_Ori (split into WT and AC). AC, aneuploid cells.