Fig. 3: Warming stimulates the C-decomposing capacity and activity of active microbial communities.

a PCoA analysis showing that the functional gene composition of activebacterial communities is significantly different between warmed (red) and control (blue) samples. b The congruence between taxonomic (triangle) and functional gene compositions (circle) of active community assessed by a Procrustes analysis optimized through a PCoA plot (red: warming samples; blue: control samples). c The congruence between DOM (triangle) and functional gene composition (circle) assessed by a Procrustes analysis optimized through a PCoA plot (red: warming samples; blue: control samples). For (b, c), The overall fit of the Procrustes transformation is reported as the M²_1,2 value. Significance is assessed using a two-sided PROcrustean Randomization Test (PROTEST)¹¹², wtih 999 permutations. d Response ratios of GeoChip signal intensities of C-decomposing genes between the warming and control samples. Red symbols represent significantly positive response ratios, while blue symbols represent significantly negative response ratios. Grey symbols represent non-significant response ratios. Each symbol represents the average ± 95% CI of four biological replicates (n = 4) of warmed or control samples. Significance is denoted as follows: *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 as determined by using the one-sided Response Ratio test¹¹⁰. No adjustments were made for multiple comparisons, and exact p-values are provided in the Source Data file. Source data are provided as a Source Data file.