Fig. 1: Hfe2-deficient mice displays severe BCB disruption.
From: The liver and muscle secreted HFE2-protein maintains central nervous system blood vessel integrity
a Schematic of the BCB assessment method using light-sheet microscopy. b 3D rendered representative light-sheet microscopic images of TR-dextran deposition in CUBIC-cleared brains (scale bars, 400 µm) and time-series quantification (mean ± s.e.m.; unpaired two-tail t-test; Hfe2fl/fl (control) n = 5, Hfe2ΔAlbCre n = 6, AAV8-GFP n = 5, AAV8-AlbCre n = 7; n representing data generated from one brain). c Representative in-vivo multiphoton images of TR-dextran at 40 mins time-point (scale bar, 50 µm) from the parietal lobes of the cerebral cortex. The normalized extravascular fluorescence (ΔF/F) intensity was plotted over time (mean ± s.e.m.; unpaired two-tail t-tests) and the groups differences were assessed with spatial leakage log-likelihood (repeated measures factorial ANOVA rendered as approximate unpaired two-tail t-test; Hfe2fl/fl n = 5, Hfe2ΔAlbCre n = 10, AAV8-GFP n = 6, AAV8-AlbCre n = 5). d Representative confocal images of fibrinogen disposition around the vessels of cerebral cortex (isolectin) of Hfe2-deficient mice (scale bar, 50 µm) and quantification (mean ± s.e.m.; unpaired two-tail t-test; Hfe2fl/fl n = 4, Hfe2ΔAlbCre n = 5, AAV8-GFP n = 5, AAV8-AlbCre n = 6). e Confocal imaging of TR-dextran deposition in brain slices from the cerebral cortex with endothelium counterstain. The same observation was made in sections from 3 AAV8-GFP and 3 AAV8-AlbCre brains. f 3D rendered representative light-sheet microscopic images of TR-dextran deposition in CUBIC-cleared brains (scale bars, 400 µm; mean ± s.e.m.; unpaired two-tail t-test; Hfe2fl/fl n = 5, Hfe2ΔActaCre n = 6). *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file (includes exact p-values).
