Fig. 2: Hfe2 -liver and -muscle knockout affect neuron number and behavior.
From: The liver and muscle secreted HFE2-protein maintains central nervous system blood vessel integrity
a NeuN staining of the same cortical area (parietal lobes) shows a significant decrease in the number of neurons (scale bar, 50 µm; mean ± s.e.m.; unpaired two-tail t-test). All mice were 6-8 weeks old when experiments were initiated. Replicates Hfe2fl/fl n = 6, Hfe2Alb-cre n = 6, Hfe2Acta-cre n = 6, and AAV8-Alb-cre n = 5, 5, 3, and 3 at weeks 1, 2, 3, and 4 respectively. b Hfe2 liver knockout mice display behavioral abnormality. Quantification of escape latency shows greater time response in Liver knockout (mean ± s.e.m.; unpaired two-tail t-test; Hfe2fl/fl n = 10 and Hfe2ΔAlb-cre n = 5). c Bar graph of marbles buried by Hfe2 KO mice (mean ± s.e.m.; unpaired two-tail t-test; Hfe2fl/fl n = 20, Hfe2ΔAlb-cre n = 10, and Hfe2ΔActa-cre n = 10). d Bar graph of three main open field test criteria for Hfe2 KO mice (mean ± s.e.m.; unpaired two-tail t-test; Hfe2fl/fl n = 10, Hfe2ΔAlb-cre n = 15, and Hfe2ΔActa-cre n = 10). *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file (includes exact p-values).
